Electrochemical evidences in oxidation of acetaminophen in the presence of glutathione and N acetylcysteine Chemical Communications (RSC Publishing) Tylenol and Glutathione: A Closer Look At Their Relationship Tylenol depletes something called glutathione. Glutathione is a master antioxidant and your first line of protection against environmental toxins. It detoxifies and protects your cells. It protects your immune system and When Good Drugs Go Bad: Acetaminophen Toxicity tl;dr pharmacy Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Myth busting, Acetaminophen version. "Avoid Acetaminophen (Tylenol) because it causes glutathione depletion". Truth?? As a pediatrician and ER doctor and as moms we're often asked about this. And we understand why: