Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Perinatal exposure to paracetamol: Dose and sex dependent effects in behaviour and brain's oxidative stress markers in progeny ScienceDirect Oxidative stress during acetaminophen hepatotoxicity: Sources, pathophysiological role and therapeutic potential ScienceDirect Acquired pyroglutamic acidosis due to long term dicloxacillin and paracetamol use BMJ Case Reports Acetaminophen Induced Hepatotoxicity: a Comprehensive Update
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glutathione depletion paracetamol Liver injury induced by paracetamol and challenges associated with intentional and unintentional use