How can I minimize liver damage while taking Tylenol (acetaminophen) in relation to glutathione levels? A guide to managing acute liver failure Cleveland Clinic Journal of Medicine Tylenol and glutathione, actually explainedhere's the bullet points: * Lots of concern raised on social media re: glutathione depletion and acetaminophen use * Glutathione is used in the metabolism of acetaminophen and Phases 1 and 2 acetaminophen metabolism. Acetaminophen consumed in Download Scientific Diagram Glutathione, Tylenol & Your Health: What You Should Know Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.