Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Tylenol And Glutathione Invigor Medical Acetaminophen induces liver injury and depletes glutathione in mice brain: Prevention by Moringa oleifera extract ScienceDirect What is Acetaminophen Uses, Dangers, Risks, & More Rubicon Please hear my heart here! There's no judgment from me, but this info is important and definitely not something widely talked about! It is very common advice parents receive to just give The gamma glutamyl pathway after glutathione depletion due to Download Scientific Diagram